Anti-Complement factor B (human)
Mouse monoclonal antibody
Cat.No. HYB 008-02
Preparation: Protein-A purified
Content: 200 µL, 1 mg/mL
Solvent: 0.01 M phosphate buffer, pH 7.4, containing 0.5 M NaCl and 15 mM sodium azide
Storage: In the dark at 4-8ºC
Factor B is a component of the alternative pathway of complement activation. The protein consists of a single polypeptide chain of molecular mass 93 kDa, and has a concentration in plasma of approximately 200 µg/ml. Factor B is cleaved by factor D into the Ba component (30 kDa) and the Bb (63 kDa). Together with C3b, Bb forms the alternative pathway C3 convertase C3bBb (1).
Complement factor B purified from human plasma and adsorbed to aluminum hydroxide gel
HYB 008-02 is specific for the Bb fragment of human factor B. Antibody binding abolishes the formation of C3bBb complexes.
Differs from that of HYB 008-06. Also differs from HYB 008-04 and HAV 005-02 but slight overlap as determined by some mutual inhibition of antibody binding
HYB 008-02 reacts strongly with human factor B. Strong reaction is seen when used as detection antibody in sandwich ELISA with a polyclonal capture antibody against factor B (e.g. DAKO A 0343). In Western blotting after SDS-PAGE, HYB 008-02 reacts with the Bb fragment of factor B in its non-reduced form only. HYB 008-02 can be used for the affinity purification of human factor B, when coupled to a suitable matrix.
| Method | Usability | Dilution guideline | References |
| ELISA | Yes | 1/32,000 | |
| Immunoblotting | Yes | ||
| Immunohistochemistry | Not determined |
The dilution guideline for ELISA is based on use as detection antibody in sandwich ELISA with a polyclonal capture antibody. Users should determine the optimal dilutions for their own purposes.
1. Kolb WP, Morrow PR, Tamerius JD (1989) Ba and Bb fragments of factor B activation: fragment production, biological activities, neoepitope expression and quantitation in clinical samples. Complement Inflamm 6:175-204.
Downloads
HYB 008-02.pdf (30.58 kB) Product specification
Complement flyer.pdf (422.22 kB) Complement flyer
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